UR-CNRFI


    CNR

    RU-CNRFI National Research Center, Florence – Institute for Plant Protection

    RU-CNRFI will be mostly involved in WP3 will also contribute with expertise in WP1, especially in connection with RU-FI. Quercus species of urban forestry interest will be the test material. Special attention will be devoted to verify if future environmental conditions will allow the antioxidant systems to check cell ROS homeostasis, so avoiding the programmed cell death. We know that biosynthesis of dihydroxy B-ring-substituted flavonoids is enhanced mostly when the activity of antioxidant enzymes is depressed, probably because of cellular redox changes. For isoprenoids, biochemical and molecular measurements will be of paramount importance, as stress-induced stimulation of volatile isoprenoids emission may have important feedback on air quality, exacerbating the formation of O3 and other urban pollutants. Specific attention will be given to the evaluation of the efficiency of the antioxidant pool and the genetic/biochemical bases of defense actions, both constitutive or induced. Functional genomics, molecular bases for resistance and tree adaption will represent another fundamental research line. Gene transcripts for enzymatic antioxidant defence (i.e. SOD, CAT, APX, POX) will be analysed by means of RT-PCR and q-PCR. For non-enzymatic defence molecules, the key steps in biosynthesis of isoprenoids and flavonoids will be investigated. About the non-enzymatic defence systems, key regulatory steps in the biosynthesis of isoprenoids and flavonoids will be analyzed. For the isoprenoid biosynthetic pathway attention will be given to transcriptional and translational limitations related to the biosynthesis of isoprene. Emission of isoprene ad its oxidation derivates will also be investigated to ascertain the reactions due to isoprene inside the leaf and in air, especially in polluted atmospheres. GC-MS analyses will be complemeneted with measurements of volatile compounds, by means of a ultra-fast and ultra-sensitive proton transfer reaction-mass spectrometry (PTR-MS).

    Project team

    Dott. Massimiliano Tattini, Dott.ssa Silvia Fineschi, Dott. Maurizio Castaldini.

    Contacts

    Dott. Massimiliano Tattini

    Istituto per la Protezione delle Piante, Consiglio Nazionale delle Ricerche

    Via Madonna del Piano, 10 50019 Sesto Fiorentino (FI) Italia

    Phone +39 055.4574038

    Fax +39 055.4574017

    e-mail m.tattini@ipp.cnr.it

    skype

    Link

    http://www.ipp.cnr.it/

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